6株鹅细小病毒的分离鉴定及VP3基因序列分析

Isolation and Identification of 6 strains of Goose Parvovirus and Analysis of VP3 Gene

关键词:  鹅细小病毒  分离鉴定  V P3基因

Key words:  Goose Parvovirus  Isolation  Identification  VP3 Gene

摘要:

为了解吉林省不同地区小鹅瘟流行情况,从吉林省磐石、白城、九台、德惠、辽源、镇赉地区鹅养殖场采集疑似小鹅瘟病料,接种SPF鹅胚后分离到6株病毒(暂命名为PSH、BCH、JLJT、DH、LY、ZL株),经PCR鉴定为鹅细小病毒。VP3基因序列分析结果显示,分离到的6株病毒VP3基因、蛋白与近年来国内流行的鹅细小病毒同源性较高,分别为93.1%～99.6%与95.9%～99.4%。其中,BCH株、JLJT株、LY株属同一分支,与浙江、扬州等地区分离的鹅细小病毒相似性较高,达到99.4%-99.6%；ZL株、DH株、PSH株属同一分支,相似性较低。试验表明,吉林省流行的鹅细小病毒与我国南方地区流行毒株同源性较高,与其他地区流行毒株存在一定差异。

Abstract:

In order to evaluate Goose Parvovirus (GPV) epodemic situation in different district of Jilin province, suspected samples were collected from poultry farm in Panshi, Baicheng, Jiutai, Dehui, Liaoyuan, Zhenlai of Jilin province, and 6 strains named respectively PSH, BCH, JLJT, DH, LY and ZL were isolated via inoculating SPF goose embryos. The detection of PCR showed that they were positive for Goose Parvovirus. The result of sequence analysis of VP3 gene showed that the 6 isolated strains were high identities with the domestic popular strains in recent years, with 93.1%～99.6% and 95.9%～99.4%. Besides that, BCH, JLJT and LY located in the same branch with zhejiang , yangzhou isolated strain from Zhejiang, Yangzhou, with high identities of 99.4%～99.6%; ZL, DH and PSH located in the same branch,with low identities. This assay indicate that the GPV epidemic strain in Jilin province was high identities with epidemic strain in the south china, and different from strains in other areas.