免疫学和分子生物学技术在猪传染性胃肠炎诊断中的应用进展

Progress on the Application of Immunology and Molecular Biology Techniques in Diagnosing of Transmissible Gastroenteritis

关键词:  猪传染性胃肠炎  免疫学技术  分子生物学技术  诊断

Key words:  transmissible gastroenteritis  immunological technique  molecular biotechnology  diagnosis

摘要:

近年来，与猪传染性胃肠炎(TGE)类似的肠道传染病日益增多，以病理组织学观察和临床症状为主要指标的传统方法已不能进行准确的判断，众多学者建立了大量的免疫学和分子生物学新方法。文章就主要免疫学技术(病毒中和实验、酶联免疫吸附试验、胶体金技术)和分子生物学技术(反转录-聚合酶链式反应、反转录环介导等温扩增技术、基于纳米颗粒和DNA探针技术的超灵敏PCR方法)在TGE诊断中的最新应用研究进行了综述，以期为进一步防控该病提供参考。

Abstract:

With the increase of viral diarrhea of pigs, it’s difficult to make accurate diagnosis for transmissible gastroenteritis (TGE) by the traditional methods depending on pathological changes and clinical symptoms. In recent years, many scholars have done a lot of research about the diagnosis and detection methods of TGE, especially in immunological methods and molecular biology techniques. Accordingly, some immunological methods (virus neutralization, ELISA, immune colloidal gold technique) and molecular biology techniques (RT-PCR, reverse transcription loop-mediated isothermal amplification, ultrasensitive nanoparticle DNA probe-based PCR assay ) developed in diagnosis of TGE were reviewed and summarized in the thesis, in order to provide reference for the prophylaxis of TGE.