| 摘要: |
| 为研究李斯特菌溶血素（LLO）对大鼠肠黏膜微血管内皮细胞（RIMVECs）分泌NO、内皮素（ET-1）的影响，以及硫酸软骨素（CS）通过调节细胞微环境保护RIMVECs的作用，将体外培养的RIMVECs分为LLO+CS组、LLO组、CS组、空白组，经过MTT比色法测定细胞生长情况，硝酸还原酶法测定细胞上清液细胞因子NO含量以及酶联免疫法检测ET-1含量，并用原位杂交方法对结果进行验证。结果表明：LLO可抑制RIMVECs增殖活性，提高NO、ET-1分泌量且高于正常水平，并导致NO/ET-1比值失衡（下降）；而CS可提高RIMVECs细胞的增殖活性，并显著上调NO/ET-1比值。由此可知，CS可通过改善LLO引起细胞因子NO和ET-1失衡而起到保护微血管内皮细胞的作用。 |
| 关键词: 硫酸软骨素 肠黏膜微血管内皮细胞 NO ET-1 LLO |
| DOI： |
| 投稿时间：2015-10-28修订日期：2015-10-29 |
| 基金项目:北京市自然科学基金A类重点项目（6061001）；北京市教委人才强教“学术创新团队”中药方剂研究（5090245） |
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| Effects of Chondroitin Sulfate on the Secretion of NO and ET-1 in Rat Intestinal Mucosa Microvascular Endothelial Cells Induced by LLO |
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| (China Institute of Veterinary Drug Control) |
| Abstract: |
| The purpose of this study was to investigate the treatment mechanism of the chondroitin sulfate (CS) on the listeriosis and the secretion of NO and ET-1 in rat intestinal mucosa microvascular endothelial cells (RIMVECs) induced by listeriolysin O (LLO). RIMVECs in culture were divided into LLO CS, LLO, CS and blank control groups. Cells were assessed proliferation by MTT assay. The Changes of NO and ET-1 level were measured by the method of nitratase, ELISA and hybridization in situ. Compared with other groups, proliferation of LLO group was very significantly decreased. The production of NO and ET-1 were enhanced for 12 h, and NO/ET-1 ratio was lower. CS could regulate imbalance of NO/ET-1 induced by LLO and improve the local microcirculation of intestine. |
| Key words: chondroitin sulfate intestinal mucosa microvascular endothelial cells NO ET-1 LLO |
