乌鳢舒氏气单胞菌的分离鉴定及药敏试验

Isolation, Identification and Drug Susceptibility of Aeromonas schubertii in Snakeheaded Fish

关键词:  乌鳢  舒氏气单胞菌  分离鉴定  药敏试验

Key words:  snakehead fish  Aeromonas schubertii  isolation and identification  drug susceptibility

摘要:

对广东省佛山市某乌鳢养殖鱼场患病的乌鳢进行病原分离，将疑似分离菌S-1株进行形态和培养特性、生化特性鉴定、PCR鉴定、药敏试验和人工感染等试验。结果表明，该分离菌为舒氏气单胞菌。药敏试验结果表明，本分离菌对壮观霉素、米诺环素、庆大霉素、氧氟沙星、诺氟沙星、头孢类等多种药物敏感。本研究为舒氏气单胞菌病细菌学检测及治疗提供了科学依据。

Abstract:

Bacterial pathogen was isolated in a snakeheaded fish farm in Foshan, Guangdong province. S-1 strain pathogenic bacterium was isolated from sick snakeheaded fish; the isolated bacterium was identified by morphological characteristics, biochemical characteristics researches, amplification of 16S rRNA by PCR and drug susceptibility test, experimental infection test. The isolated bacterium was confirmed as Aeromonas schubertii. Drug susceptibility test results showed that the isolated bacterium were sensitive to spectinomycin, minocycline, gentamycin, ofloxacin, norfloxacin and cephalosporin etc. The results provided scientific bases for detection and treatment of Aeromonas schubertii.