ELISA法检测种蛋卵黄中禽网状内皮组织增生症病毒抗体的研究

Study on the ELISA Detection for Avian Reticuloendotheliosis Virus Antibody in Egg Yolk

关键词:  网状内皮组织增生症病毒  ELISA  卵黄抗体

Key words:  REV  ELISA  egg yolk antibody

摘要:

为研究种蛋卵黄中禽网状内皮组织增生症病毒（REV）抗体的检测方法，运用IDEXX公司的REV抗体ELISA检测试剂盒，比较了不同提取方法、不同稀释倍数、不同洗液对卵黄中REV抗体检测结果的影响。结果显示：当用直接提取的卵黄液做300倍稀释检测，用含0.05%吐温-20的蒸馏水洗涤时，卵黄抗体的S/P比值与相应鸡群的血清抗体的S/P比值吻合度最高。应用本方法分别对50枚SPF种蛋和50枚普通鸡蛋进行检测，SPF种蛋均为REV抗体阴性；普通鸡蛋有4枚为REV抗体阳性，阳性率为8%。实验表明，种蛋卵黄ELISA 抗体检测法可以代替传统血清抗体检测，为SPF鸡群的REV感染监测奠定了基础。

Abstract:

In order to detect avian reticuloendotheliosis virus antibody in egg yolk,the IDEXX ELISA kit for REV antibody detection was used, and the effects of different extraction methods, different dilution ratios and different distill water of egg yolk on detection results were compared. The result showed that the S/P ratio of egg yolk antibody was closer to that of serum ELISA antibody of the same chicken group when the egg yolk antibody was directly extracted from eggs and was diluted into 1∶300, and distill water containing 0.05% Tween-20 was used. Using this ELISA method, 50 SPF eggs and 50 ordinary eggs were tested.The result showed that all of SPF eggs were REV antibody negative;four ordinary eggs were REV antibody positive with the positive rate of 8%.This study showed that egg yolk antibody ELISA detection method could replace the traditional serum antibody detection, and would lay the foundation for Rev infection monitoring of SPF chickens.