耐受自身代谢产物及耐二甲基亚砜红霉素高产菌株的选育

Screening of high yield Erythromycin producing strain by combined drug resistant mutationSHEN Xiao-Jing, ZHANG Ping*, SHI Yan-Peng #$NL(Ningxiatairui Pharmaceutical Co., Ltd. strains Institute, Yinchuan, Ningxia 750101,China)

关键词:  红霉素  耐受自身代谢产物  二甲基亚砜  遗传稳定性  ARTP

Key words:  Erythromycin  self--secondary metabolites resistant  dimethyl sulfoxide(DMSO)  Hereditary stability  Atmospheric and Room Temperature Plasma.

摘要:

【】为了提高红霉素发酵单位,采用了筛选自身代谢产物突变株的方法,将耐受突变株进行ARTP诱变和耐受高浓度二甲基亚砜(DMSO)处理。结果显示,通过在36h向红色糖多孢菌发酵瓶中添加30,000ug/mL红霉素,筛得化学效价比对照提高22.4％的菌株。最适的DMSO添加时间及剂量为发酵48h时添加0.2％。DMSO可提高发酵单位10.1％。

Abstract:

To improve the production of Erythromycin,the mutated strains were seleeted by use of self--secondary metabolites.The mutant resistanting 30,000ug/ml of erythromyein was further treated by ARTP and DMSO.The result showed that,the chemical titre of erythromycin was increased by 22.4％of the control when 30,000ug/mL erythromycin was added into the medium at 36h of fermentation of Saccharopolyspora erythraea.The suitable volume fraction and adding time of DMSO were 0.2％ at 48h of fermentation.Adding DMSO could increase the yield of erythromycin by 10.1％.