硫酸头孢喹肟乳房（干乳期）注入剂在奶牛乳汁中的残留消除研究

Residual Study of Cefquinome Sulfate Intramammary Infusion (Dry Period) in Milk

关键词:  头孢喹肟  牛奶  残留  超高效液相色谱-串联质谱法

Key words:  cefquinome  milk  residue  UPLC-MS/MS

摘要:

建立了牛奶中硫酸头孢喹肟残留超高效液相色谱-串联质谱检测方法(UPLC-MS/MS)。选取18头刚进入干乳期的健康奶牛，随机分为 6 个组，分别于预产期前的42、35、28、21、14和7 d进行乳房注入硫酸头孢喹肟乳房注入剂（3 g∶150 mg/支），只用药一次，产犊后采集奶样。结果表明：预产期前7 d给药，奶牛产犊 72 h后采集的奶样中头孢喹肟残留量降至MRL（20 μg/kg）附近；预产期前14 d和21 d给药，奶牛产犊12 h后采集的奶样中头孢喹肟残留量降至MRL以下，均低于检测限；预产期前 28、35 和 42 d给药，所有采集奶样的头孢喹肟残留量均低于检测限。建议在预产期前 7、14 和 21 d 给药硫酸头孢喹肟乳房注入剂的弃奶期分别为产犊后5、1和0 d。

Abstract:

The high performance liquid chromatography and tandem mass spectrometry method (UPLC-MS/MS) was established for the residual study of cefquinome sulfate in milk.18 healthy cows that just entering the dry period were selected and were randomly divided into 6 groups. The 6 groups of cow were respectably administrated cefquinome sulfate intramammary infusion (dry period) once in mammary region at the dose of 3 g for each region and their milk were collected after calving. The results demonstrated that: administrated 7 days before preterm birth, concentration of cefquinome in milk collected 72h after calving decreased to about MRL; administrated 14 and 21 days before preterm birth, concentration of cefquinome in milk collected 12h after calving dropped below MRL, all below the detection limit; administrated 28、35 and 42 days before preterm birth，concentration of cefquinome in all the milk were below the detection limit. It is suggested that the milk withholding period were respectably 5, 1 and 0 days that administrated cefquinome sulfate intramammary infusion7, 14 and 21 days before preterm birth.