高效液相色谱法测定鸡肌肉中地克珠利、妥曲珠利及其代谢物的残留量

Determination of residues of diclazuril, toltrazuril, toltrazuril sulphone and toltrazuil sulphoxide in chicken muscle by HPLC

关键词:  高效液相色谱法  地克珠利  妥曲珠利  代谢物  鸡肌肉  残留量

Key words:  HPLC  diclazuril  toltrazuril  metabolite  chicken muscle  Sresidues

摘要:

建立了一种高效液相色谱法同时测定鸡肌肉中地克珠利、妥曲珠利、妥曲珠利砜及妥曲珠利亚砜残留量的方法。试样经乙腈涡旋提取,正己烷脱脂,经碱性氧化铝小柱净化,浓缩,流动相定容,高效液相色谱分离,紫外检测器检测,以保留时间定性,以峰面积定量。结果表明：该方法在浓度0.05-5μg/mL范围内线性良好,相关系数r均大于0.999。在50μg/kg-500μg/kg添加浓度范围,方法回收率在60%-110%之间,批内和批间变异系数均小于15%。方法简便、灵敏度高、稳定性好。

Abstract:

To establish a high performance liquid chromatography(HPLC)method to determinate the residues of diclazuril, toltrazuril, toltrazuril sulphone and toltrazuil sulphoxide in chicken muscle simultaneously. The samples were extracted with acetonitrile, defatted with n-hexane, followed by an Alumina column clean-up procedure, evaporated to dryness and dissolved the residues with mobile phase. HPLC was employed for separation and UV detector was used to test directly. Qualitative analysis was done by retention time and quantitative analysis by peak area respectively. Results: A good linear relationship was obtained in the range of 0.05-5 μg/mL with the correlation coefficients all above 0. 999. The average recoveries ranged from 60% to 110% of the fortified concentration at 50-500 μg/kg levels, and the variable coefficients of within-runs and between-runs were all less than 15%. The method is simple, sensitive and stable.