| 摘要: |
| 通过PCR技术，从自行分离的鸭甲肝病毒JS株基因组中扩增出病毒衣壳蛋白VP1完整基因片段，并对该片段进行序列测定及分析。结果显示JS-VP1与DHAV-A的VP1基因序列酸核苷酸同源性在62.0%～64.4%之间，与DHAV-B的VP1基因序列酸核苷酸同源性在63.6%～63.8%之间，与DHAV-C的VP1基因序列酸核苷酸同源性在92.3%～98.5%之间，分析表明JS株为鸭甲肝病毒基因C型，血清型分型为韩国新型。进化树表明JS株与SD02株的亲缘关系较进，进一步说明VP1基因的核苷酸序列是DHAV基因组中的高度可变区，可作为DHAV基因型研究的标记，分离毒株为DHAV的防治及疫苗的设计奠定了基础。 |
| 关键词: 鸭甲肝病毒 VP1基因 序列分析 |
| DOI： |
| 投稿时间：2014-10-14修订日期：2014-12-10 |
| 基金项目: |
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| Clone and Sequence Analysis of VP1 Gene of Duck Hepatitis A Virus |
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| (Harbin Pharmaceutical Group Co Ltd Biological Vaccine) |
| Abstract: |
| VP1 gene of duck hepatitis A virus was amplified from the gene by PCR, and then sequenced. The result of sequence analysis showed that the gene of VP1 of JS strain and DHAV-A shared identities of 62.0%～64.4%, DHAV-B shared identities of 63.6%～63.8%, and DHAV-C shared identities of 92.3%～98.5% which showed that JS strain was DHAV- C and a new serotype strain in Korea. Phylogenetic analysis showed that it had the closest relationship with SD02 strain．This conclusion further illustrated that the nucleotide sequence of VP1 gene was highly variable area of DHAV, and could be used as DHAV genotype research. The isolate will lay a theoretical basis for the disease prevention and vaccine design. |
| Key words: DHAV VP1 gene sequence analysis |
