| 摘要: |
| 为制备猪瘟特异性抗体,将猪瘟兔化弱毒E2基因与Mels和6His基因序列融合后克隆入pFastBac1质粒载体,经转座、转染后构建重组杆状病毒,并用纯化的表达产物E2蛋白免疫大耳白兔,采血、经Protein A纯化后获得猪瘟特异性抗体。结果成功获得能分泌猪瘟E2蛋白的重组杆状病毒rBacVE2,纯化的E2蛋白,可用于制备猪瘟抗体,为猪瘟病毒荧光抗体染色液的制备奠定了基础。 |
| 关键词: 猪瘟病毒 E2基因 杆状病毒表达,抗体制备 |
| DOI： |
| 投稿时间：2014-10-09修订日期：2014-12-11 |
| 基金项目: |
|
| Construction of Recombinant Baculovirus Expressing HCLV E2 Gene and Antibody Preparation |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| In order to prepare antibody for detection of classical swine fever virus(CSFV), the E2 gene of vaccine strain hog cholera lapinised virus(HCLV) and Mels, 6His sequence were cloned into vector pFastBac1, through thransposon and transfection, a recombinant baculovirus expressing HCLV E2 Gene was constructed. E2 protein was purified and immunized in rabbits, serum was purified through Protein A, confirmed by indirect FA, the antibody against CSFV was prepared. |
| Key words: CSFV, E2 gene, BEVS, Antibody Preparation |
