复方黄芪对雏鸡免疫机能的影响

The Effects of Compound Astragalus Preparations on the Immunity Function in Chickens

关键词:  复方黄芪  雏鸡  免疫器官指数  γ-IFN  IgG  IL-2

Key words:  Compound Astragalus  chicken  immune organ index  IFN-γ  IgG  IL-2

摘要:

为探讨复方黄芪制剂对雏鸡免疫机能的影响，将200只7日龄农大3号雏鸡随机分成Ⅰ组（健康对照组）、Ⅱ组（感染对照组）、Ⅲ组（中药治疗组）和Ⅳ组（中药预防组）。Ⅰ组不感染，不给药，Ⅱ组、Ⅲ组于7日龄时按按100 ELD50剂量滴口感染鸡IBDV，Ⅲ、Ⅳ组于8日龄按20 g/kg体重，口服复方黄芪制剂，每天2次，连续灌服6 d。然后在14、17、20日龄时各组取8只，分别称重后自心脏采血1.0 mL，制备血清，用于IFN-γ、IgG、IL-2的测定。之后扑杀鸡只，摘取法氏囊、脾脏、胸腺，用于计算免疫器官指数。结果如下：中药预防组与健康对照组相比，能明显提高雏鸡的免疫器官指数，提高血清中IFN-γ、IgG、IL-2的含量；中药治疗组与感染对照组相比，前者明显能修复损伤的免疫器官，恢复正常生长水平，同时血清中IFN-γ、IgG、IL-2的含量，也均显著或极显著高于后者。提示复方黄芪制剂能提高雏鸡的免疫机能，对鸡IBD有较好的防治作用。

Abstract:

To investigate the effects of compound astragalus preparations on the immunity function in chickens，the 200 seven day-old Nongda 3rd chickens were randomly divided into group Ⅰ( healthy control group ), group Ⅱ ( infected control group ), group Ⅲ ( treatment group ) and group Ⅳ (prevention group ). Group Ⅰwas not infected and not administed , the chickens in group Ⅱ and group Ⅲ were infected with 100 ELD50 dose IBDV by mouth drip when they were 7 days of age, group Ⅲ and group Ⅳ were treated with compound astragalus preparations by 20 g/kg each at eight day-old, two times a day, continuously 6 days. Then, when 14,17,20 day-old ,we take 8 chickens from each group, collected blood 1 mL from the heart after weighed, and prepared the serum for detection of IFN-γ, IgG and IL-2. Last culled the chickens , removed the bursa fabricius, spleen , thymus, calculated the immune organ index. The results are as follows: the prevention group compared with the healthy control group, the compound astragalus preparations can significantly improve the immune organ index on chickens, raise the serum IFN-γ, IgG and IL-2. The treatment group compared with the infected control group, the former was able to repair the damaged immune organ, and restore the normal growth levels, while the serum levels of IFN-γ, IgG and IL-2, also were significantly or very significantly higher than the latters. This study shows that the preparation of compound astragalus can improve the immunity function in chickens, and it has a good preventive effect on the chicken’s IBD.