| 摘要: |
| 为检测不同毒力结核分枝杆菌感染巨噬细胞后细胞因子转录水平的变化，构建IL-6、IL-10、TNF-α重组标准质粒，建立了实时荧光定量PCR标准曲线。应用该方法对不同毒力结核分枝杆菌感染RAW264.7巨噬细胞6个时间点细胞因子的转录水平进行分析，结果显示H37Rv组、BCG组相比于对照组刺激后均使三种细胞因子的转录水平发生变化，其中IL-6和TNF-α发生明显变化。本试验通过对不同时间点细胞因子转录水平研究的分析为结核分枝杆菌对巨噬细胞凋亡机制的研究提供新思路。 |
| 关键词: 结核分枝杆菌 RAW264.7细胞 荧光定量PCR 细胞因子 |
| DOI： |
| 投稿时间：2014-09-10修订日期：2014-11-03 |
| 基金项目:国家科技支撑计划(2011BAI03B02-1)；吉林省科技厅科技支撑计划(20120225) |
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| Establishment and application a standard curve of Quantitative Real-time PCR for cytokine transcript levels after infected by different virulence Mycobacterium tuberculosis at different time point |
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| (College of Animal Science Technology,Jilin Agricultural University) |
| Abstract: |
| IIn order to detect the change of cytokine transcript levels after infected by Mycobacterium tuberculosis, buildingrecombinant standard plasmids of IL-6, IL-10,TNF-α, a quantitative real-time PCR standard curve was established for Cytokine transcript levels.Application of the method was used to analyze Cytokine cytokine transcript levels that different virulenceMycobacterium tuberculosisinfecting RAW264.7 macrophages at six time points.Comparison with the control group,H37Rv and BCG made changes in transcript levels of these three cytokines, whlie the IL-6 and TNF-α have more obvious varies.This study provided new ideas of mechanismbetween Mycobacterium tuberculosis and macrophages through different time points cytokines transcriptional level. |
| Key words: Mycobacterium tuberculosis RAW264.7 cell fluorescence quantitative PCR Cytokine |
