| 摘要: |
| 建立了盐酸左旋咪唑粉中盐酸左旋咪唑含量的高效液相色谱测定法。以0.05 mol/L磷酸二氢钾（用三乙胺调节pH值为7）-乙腈（80∶20）为流动相，盐酸左旋咪唑经C18色谱柱分离，在214 nm波长下检测。试验结果表明，盐酸左旋咪唑在0.1～2.0 mg/mL范围内线性关系良好，R2=0.9995，平均回收率为101.3%(n=6)，RSD为0.6%。本方法简便、准确、重现性好，可更好的控制盐酸左旋咪唑粉的质量。 |
| 关键词: 盐酸左旋咪唑粉 盐酸左旋咪唑 高效液相色谱法 |
| DOI： |
| 投稿时间：2014-08-27修订日期：2014-12-02 |
| 基金项目: |
|
| Determination of Levamisole Hydrochloride in Levamisole Hydrochloride Powder by HPLC |
|
| (Henan Provincial Supervising Agency of Veterinary) |
| Abstract: |
| The method for determination of levamisole hydrochloride in levamisole hydrochloride powder by HPLC was developed. The analyte was separated on C18 chromatographic column using a mobile phase of potassium dihydrogen phosphate (0.05 mol/L,pH value adjusted to 7.0 with triethylamine)-acetonitrile (80∶20), and detected at the wavelength of 214 nm. The method was validated for specificity,accuracy,linearity, precision and robustness.The results showed that a good linear relationship was accepted in the range of 0.1～2.0 mg/mL (R2=0.9995), and the average recovery was 101.3% (n=6), and the RSD value was 0.6%. This method was suited to control the quality of levamisole hydrochloride powder. |
| Key words: levamisole hydrochloride powder levamisole hydrochloride HPLC |
