| 摘要: |
| 为快速检测猪繁殖与呼吸综合征病毒，本研究应用抗猪繁殖与呼吸综合征病毒单克隆抗体PRRSV-3D10株和4H11株分别作为胶体金标记物和诊断抗体，羊抗鼠IgG作为质控线，制备胶体金免疫层析抗原检测试纸。该试纸卡具有良好的特异性、敏感性、重复性和稳定性，与PCR方法对比总符合率为93.3%。研究显示，本试纸卡能够快速、准确检测猪繁殖与呼吸综合征病毒。检测结果可以为临床诊断提供参考。 |
| 关键词: 猪繁殖与呼吸综合征病毒 抗原 胶体金试纸 |
| DOI： |
| 投稿时间：2014-07-04修订日期：2014-08-28 |
| 基金项目: |
|
| Development of Colloidal Gold Strip for Detecting Porcine reproductive and respiratory syndrome virus |
|
| (WuHan Chopper Biological Co.,Ltd.) |
| Abstract: |
| For rapid detection of porcine reproductive and respiratory syndrome virus, two kinds of anti-PRRSV monoclonal antibody 3D10 and 4H11 used respectively as colloidal gold-labeled antibody and diagnostic antibody, goat anti-mouse IgG as control line, a new colloidal gold strip was developed according to the principle of colloidal gold immunochromatography. The test strip showed good specitivity, sensitivity and repeatability, The coincidence of the strip was 93.3% compared to the PCR test kit. The research provided the probability for rapid diagnosis of disease caused by PRRSV considering it was simple, rapid and suitable for field testing. |
| Key words: PRRSV antigen colloidal gold strip |
