| 摘要: |
| 建立了氟喹诺酮类药物可溶性粉中非法添加物利巴韦林检测的UPLC-MS/MS方法。样品经提取稀释后，采用ACQUITY UPLCR BEH amide色谱柱为分离柱，质谱正离子扫描测定。结果显示，利巴韦林在1~50 ng/mL的范围内线性关系良好（R2=0.9984）；样品检出限为0.5 mg/g，定量限为2 mg/g。在40、50、60mg/g添加水平的回收率为90%～110%，批内批间变异系数均小于10%。本方法快速、灵敏、重现性好，适用于氟喹诺酮类药物可溶性粉中非法添加利巴韦林的检测。 |
| 关键词: 利巴韦林 氟喹诺酮类药物 超高效液相-串联质谱法 |
| DOI： |
| 投稿时间：2014-05-05修订日期：2014-06-13 |
| 基金项目: |
|
| Determination of Ribavirin Illegally Added in the FQs soluble powder by UPLC-MS/MS |
|
| (Shandong Provincial Veterinary Medicine Supervision Institute) |
| Abstract: |
| A method was established for determination of ribavirin added in FQs soluble powder by UPLC-MS/MS. The ribavirin were extracted from diluted samples and separated by ACQUITY UPLCR BEH amide. The ribavirin was analyzed by UPLC-MS/MS with positive ion mode.The results showed that the good linear range was 1 to 50 ng/mL（R2=0.9984）. The detection limit was 0.5mg/g，and the quantitation limit was 2 mg/g. The analysis of added 40、50、60mg/g ribavirin showed that the recoveries ranged from 90% to 110% and the relative standard deviations were below 10%. The method was speedy, sensitive and reproducibility，suitable for the determination of ribavirin added in FQs soluble powder . |
| Key words: Ribavirin FQs Veterinary Medicine UPLC-MS/MS |
