高效液相色谱法测定穿心莲注射液的含量

Determination of Chuanxinlian Injection by HPLC

关键词:  高效液相色谱法  穿心莲注射液  穿心莲内酯  脱水穿心莲内酯

Key words:  HPLC  Chuanxinlian injection  andrographolide  dehydroandrographolide

摘要:

建立了穿心莲注射液中穿心莲内酯和脱水穿心莲内酯含量测定的高效液相色谱方法。采用Waters XBridge C18色谱柱(150 mm×4.6 mm, 5 μm)，甲醇：水（65∶35）为流动相，流速为1.0 mL/min，穿心莲内酯波长为225 nm，脱水穿心莲内酯波长为254 nm，柱温30 ℃。穿心莲内酯和脱水穿心莲内酯均在0.01~0.2 mg/mL浓度范围内线性关系良好。系统精密度分别为0.35%和0.30%，平均回收率分别为96.57%和97.98%。结果表明，该方法简单可行、重复性好，可用于穿心莲注射液中有效成分的含量测定。

Abstract:

A HPLC method was established for the determination of andrographolide and dehydroandrographolide in Chuanxinlian injection. A Waters XBridge C18 chromatographic column (150 mm×4.6 mm, 5 μm) was used, with the mobile phase of methanol-water(65∶35) at the flow rate of 1.0 mL/min. The detection wavelength of andrographolide and dehydroandrographolide were respectively 225 nm and 254 nm, and the column temperature was 30 ℃. Both andrographolide and dehydroandrographolide had good linearity within the concentration range of 0.01~0.2 mg/mL. The system precision were 0.35% and 0.30%, and the average recovery were 96.57% and 97.98% respectively. The method is simple, feasible and repeatable, it can be used as quality control for Chuanxinlian injection.