30味中药提取物与环丙沙星联用对猪源链球菌体外抑菌作用研究

Inhibitory effect of combination of thirty Chi-nese herbs extract with Ciprofloxacin against Streptococcus suis in vitro

关键词:  猪源链球菌  中草药  环丙沙星  抑菌作用  活性成分

Key words:  Streptococcus suis  Chinese herbs  Ciprofloxacin  antibiotics effect  Active ingredients

摘要:

为筛选与环丙沙星联用对猪源链球菌具有协同抑菌作用的中草药活性成分,采用试管二倍稀释法测定30味中草药醇提物、水提物的最低抑菌浓度(MIC),采用棋盘稀释法测定30味中草药醇提物、水提物与环丙沙星联用对猪源链球菌的部分抑菌浓度(FIC),采用有机溶剂萃取法初步追踪与环丙沙星联用具有协同抑菌作用的中草药活性成分,结果显示,所选30味中草药的水提物、醇提物对猪源链球菌均具有一定的抑菌作用,佩兰、黄连、枳壳、郁金等中草药醇提物、水提物对猪源链球菌(pf-cc)抑菌活性较强,苍术水提物等对猪源链球菌的抑菌作用较弱；佩兰醇提物与环丙沙星联用对猪源链球菌的协同抑菌作用最强；佩兰醇提物的乙酸乙酯萃取物、氯仿萃取物与环丙沙星联用对猪源链球菌分别具有协同、相加抑菌作用。

Abstract:

To screen active ingredients of Synergistic inhibitory effect in vitro of Chinese medicine herbs combined with Ciprofloxacin against Streptococcus suis. Minimum inhibitory concentration (MIC) of water extract and alcohol extract of thirty Chinese herbs against Streptococcus suis in vitro were determined by tube two-double dilution method. Fractional inhibitory concentrations (FIC) of water extract and alcohol extract of thirty Chinese herbs combined with antibiotics against Streptococcus suis were determined by constant checkerboard dilution method. Synergistic inhibitory effect of Chinese herbs with active ingredients were initial tracked by organic solvent extraction method.The results showed that water extract and alcohol extract of thirty medicine herbs have antibactierial effect against Streptococcus suis in certain extent. It’s shown that some extracts such as alcohol extract,water extract of Eupatorium fortunei Turcz,berberine,Citrus aurantium,Turmeric. have strong antibactierial activity on Streptococcus suis in vitro. And the antibactierial activity of some extracts such as water extract of Atractylodes lancea have weaker inhibitory effect on Streptococcus suis in vitro. Inhibitory bacterium additive effect was existed in the alcohol extract of Eupatorium fortunei Turcz combined with Ciprofloxacin.