高效液相色谱法测定芩黄颗粒中甘草酸的含量

Determination of Glycyrrhizic acid in Qinhuang Granula by HPLC

关键词:  芩黄颗粒  甘草酸  高效液相色谱法

Key words:  Qinhuang Granula  Glycyrrhizic acid  HPLC

摘要:

为了建立芩黄颗粒中甘草酸的含量测定方法并测定其含量，采用高效液相色谱法（HPLC）测定芩黄颗粒中甘草酸的含量，采用C18色谱柱，以甲醇-0.2mol/l醋酸铵（65：34，醋酸调节pH值至4.5）为流动相，流速为1.0ml/min，检测波长为250nm。结果显示甘草酸在0.499μg～7.976μg之间呈现良好的线性关系，r=0.9999；平均加样回收率为99.3%，RSD=0.59%（n=6）。本方法重复性好，能准确测定芩黄颗粒中甘草酸的含量。

Abstract:

To establish the method for determining of glycyrrhizic acid in Qinhuang Granula by HPLC, C18 column was used with the mobile phase consisted of methanol-0.2mol/l ammonium acetate (65:34, adjusted pH to 4.5 with CH3COOH), the flow rate was 1.0 ml/min, the detection wavelength was 250 nm. The calibration curve was linear at a range of 0.499~7.977μg for glycyrrhizic acid (r=0.9999), the average recovery was 99.3% and RSD was 0.59%(n=6). The method was accurate with a good reproducibility and can be used as a quantitative analysis of Glycyrrhizic acid in Qinhuang Granula.