| 摘要: |
| 为了对车前子提取物耐药抑制作用进行研究，采用棋盘法测定车前子乙醇提取物与环丙沙星的联合作用，二者联合应用对大肠杆菌耐药菌株具有较好的抗菌作用。通过环丙沙星蓄积动力学试验检测车前子乙醇提取物对鸡源大肠杆菌外排泵的影响，结果表明，车前子乙醇提取物能增加环丙沙星在细胞内的蓄积量，抑制耐药大肠杆菌对抗生素的主动外排作用。三氯甲烷萃取物对大肠杆菌的药物外排具有一定的抑制作用，但其抑制作用不如乙醇提取物。 |
| 关键词: 车前子 大肠杆菌 耐药 抑制 |
| DOI： |
| 投稿时间：2013-11-25修订日期：2014-05-04 |
| 基金项目:吉林省教育厅“十二五”科学技术研究项目（吉教科合字［2013］第67号）；吉林农业大学科研启动基金资助项目（201220） |
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| The Mechanism of Resistance Inhibition of Plantago asiatica L. Extracts to Escherichia coli |
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| (College of Animal Science and Technology,Jilin Agricultural University;China) |
| Abstract: |
| To evaluate the antibiotic effects of ciprofloxacin combined with extracts of Plantago asiatica L. against Escherichia coli using the checkerboard method. The FIC index was calculated according to MIC (Minimum inhibitory concentrations) results. Results suggested the additivity of ciprofloxacin combined with Plantago asiatica L. The influence on efflux pump was assessed by kinetics evaluation of ciprofloxacin in the dynamics of accumulation. The alcohol extracts of Plantago asiatica L. increased the cellular accumulation of ciprofloxacin. Chloroform extracts effectively inhibited efflux pump in J strain of Escherichia coli. |
| Key words: Plantago asiatica L. Escherichia coli drug resistance inhibition |
