恩诺沙星分子印迹聚合物的制备及表征

Preparation and Characterization of Molecularly Imprinted Polymer Microspheres for Recognition of Enrofloxacin

关键词:  分子印迹聚合物  恩诺沙星  沉淀聚合法

Key words:  molecularly imprinted polymers  enrofloxacin  precipitation polymerization

摘要:

以恩诺沙星（ENRO）为模板分子，甲基丙烯酸（MAA）为功能单体，采用沉淀聚合法合成了ENRO分子印迹聚合物（MIPs）纳米微球，优化了模板与功能单体的比例、溶剂种类及溶剂用量，并对MIPs进行了表征。结果表明，MIPs微球粒径（190-290 nm）大于非印迹聚合物（NIPs）微球粒径；同时Scatchard 分析表明，在研究的浓度范围内MIPs存在两个不同的结合位点，其离解平衡常数Kd与最大表观结合量Qmax分别为Kd1 =0.2313 g/L，Qmax1 =34.7540 mg/g，Kd2 =0.5841 g/L， Qmax2 =57.4298 mg/g；与环丙沙星（CIP）和氧氟沙星（OFL）相比，MIPs对ENRO表现出更强的特异性吸附能力。

Abstract:

Enrofloxacin (ENRO) was taken as a template, and methacrylic acid (MAA) was taken as a monomer. The microspheres of the molecularly imprinted polymer (MIPs) were prepared by precipitation polymerization. We determined the ratio of the template and the monomer, solvent species and solvent usage via experiment. The MIPs were characterized. The results showed that MIPs microspheres were an particle size of 190-290 nm, greater than the non-imprinted polymeric microspheres (NIPs). Analysis of the Scatchard plot revealed that there were two kinds of binding sites in MIPs and the dissociation constant (Kd) and apparent maximum binding capacity (Qmax) of MIPs were Kd1 =0.2313 g/L, Qmax1 =34.7540 mg/g for high affinity binding sites and Kd2 =0.5841 g/L, Qmax2 =57.4298 mg/g for low affinity binding sites, respectively. The specific absorption capacity of MIPs to ENRO is higher than those of ciprofloxacin (CIP) and ofloxacin (OFL).