20味中草药与抗生素联用对猪源链球菌体外抑菌作用的分析

Antibacterial effect of combination of twenty kinds of Chinese herbs with Ciprofloxacin against Streptococcus suis in vitro

关键词:  猪源链球菌  中草药  抗生素  联合用药  部分抑菌浓度

Key words:  Streptococcus suis  Chinese herbs  antibiotics  drug combination  fractional inhibitory concentration

摘要:

为观察中草药与抗生素联用对猪源链球菌的体外抑菌效果，采用试管二倍稀释法测定20味中草药水提物、醇提物的最低抑菌浓度（MIC），采用常量棋盘稀释法测定20味中草药水提物、醇提物与抗生素联用对猪源链球菌的部分抑菌浓度（FIC），结果显示，所选20味中草药的水提物、醇提物对猪源链球菌均具有一定的抑菌作用,瓜蒌醇提物等对猪源链球菌的体外抑菌作用较强；败酱草水提物等对猪源链球菌的体外抑菌作用较弱。瓜蒌醇提物、乌梅醇提物、乌梅水提物与环丙沙星联合用药的抑菌作用表现为相加作用，板蓝根醇提物等与环丙沙星联合用药的抑菌作用表现为无关作用。

Abstract:

To observe the antibacterial effect in vitro of Chinese medicine herbs combined with antibiotics against Streptococcus suis. Minimum inhibitory concentration (MIC) of water extract and alcohol extract of twenty Chinese herbs against Streptococcus suis in vitro were determined by tube two-double dilution method. Fractional inhibitory concentrations (FIC) of water extract and alcohol extract of twenty medicine herbs combined with antibiotics against Streptococcus suis were determined by constant checkerboard dilution method. The results showed that water extract and alcohol extract of twenty medicine herbs have antibactierial effect against Streptococcus suis in certain extent. It’s shown that some extracts such as alcohol extract of Trichosanthes kirilowii maxim have strong antibactierial effect on Streptococcus suis in vitro. And the antibactierial effect of some extracts such as water extract of Patrinia were low on Streptococcus suis in vitro. Inhibitory bacterium additive effect was existed in the alcohol extract of Trichosanthes kirilowii maxim, alcohol extract of smoked plum and water extract of smoked plum combined with Ciprofloxacin. Inhibitory bacterium unrelated effect was existed in some extracts such as alcohol extract of Isatidis Radix combined with Ciprofloxacin.