恩诺沙星注射液中非法添加磺胺嘧啶HPLC-PDA检查法的建立

Determination of Sulfadiazine Illegally Added in the Enrofloxacin Injection by HPLC-PDA

关键词:  磺胺嘧啶  恩诺沙星注射液  HPLC-PDA

Key words:  sulfadiazine  the enrofloxacin injection  HPLC-PDA

摘要:

对恩诺沙星注射液中非法添加磺胺嘧啶的检测方法进行了研究。通过比较受试样品中未知峰与对照品色谱图保留时间的一致性和光谱指数图的匹配，建立了HPLC-PDA检查法，并进行了相应的方法学研究。结果显示磺胺嘧啶的回收率为99.5%，RSD=0.4%；线性方程为Y=873577x 98154，R2=1，检测限为5ug/ml，表明该检测方法准确可靠,可用于恩诺沙星注射液中非法添加磺胺嘧啶的检测。

Abstract:

To identify a method for the test of sulfadiazine illegally added in the enrofloxacin injection, and the merchant sample was researched. HPLC-PDA method was established for the determination of the preliminary screening, and sulfadiazine was last identified by comparation the rentention time and the spectral graph matching of the reference chromatogram and spectrum. Sulfadiazine the average recoveries was 99.5%,RSD=0.4%, linear equation y=873577x 98154,R2=1,the limit of was5ug/ml. The method is accurate and reliable, and can be used in the test of sulfadiazine mixed in the enrofloxacin injection。