鸡黄病毒灭活疫苗的研制及免疫效果研究

Preparation and Protective Efficacy of Inactivated Vaccine of Chicken Flavivirus

关键词:  鸡黄病毒  灭活疫苗  免疫效果测定

Key words:  chicken flavivirus  inactivated vaccine  protective efficacy

摘要:

鸡黄病毒FQ-C1株经SPF鸡胚连续传40代后，其尿囊液毒的ELD50达到1?10－4.33/0.1 mL。将该病毒第40代SPF鸡胚液毒灭活后制备成油乳剂灭活疫苗，SPF雏鸡安全性试验和蛋鸡安全性试验表明该疫苗的安全性好，无不良影响。以该疫苗一次单剂量（1 mL/只）免疫开产蛋鸡28 d后攻以鸡黄病毒强毒测定疫苗的保护效果，结果显示疫苗免疫组较未免疫组蛋鸡的产蛋率高出75.8%，产蛋保护率达93.2%以上，表明本研究制备的鸡黄病毒灭活疫苗安全性好，且具有良好的免疫原性，免疫后可有效抵抗鸡黄病毒所致的产蛋骤降。

Abstract:

After serially passaged in specific pathogen free embryonated chicken eggs, the chicken flavivirus strain FQ-C1 was stable at a titer of 1×104.33 ELD50 per 0.1 mL after 40 passages. Inactivated with formaldehyde, the passaged virus of strain FQ-C1 emulsified with mineral oil adjuvant in an appropriate ratio, and then inactivated vaccine is made. We test the biosecurity of the inactivated oil-emulsion vaccines through immunization in 1-day-old chicken and laying hens. The results showed that animals immunized with the inactivated vaccines appear no adverse effects. Twenty-eight days after administration of inactivated vaccine with a dose of 1 mL per chicken, the laying hens attacked by a virulent chicken flavivirus. The results showed that the laying rate of hens immunized with vaccine was 75.8% higher than that of control group, and the protective efficacy of the inactivated vaccine on egg production was no less than 93.2%. All of the results indicated that the inactivated oil-emulsion vaccine had good immunogenicity, which could effectively resist egg-dropping caused by chicken flavivirus.