鸡传染性法氏囊病活疫苗(B87株)病毒含量细胞间接免疫荧光方法的建立

Development of cellular immunofluorescence method for evaluation of living infectious bursal disease vaccine (B87 strain)

关键词:  鸡传染性法氏囊病中等毒力活疫苗(B87株)  细胞间接免疫荧光方法  病毒含量测定

Key words:  infectious  bursal disease  living vaccine (B87 strain), cellular  immunofluorescence, evaluation

摘要:

采用Vero细胞建立鸡传染性法氏囊病中等毒力活疫苗(B87株)病毒含量细胞间接免疫荧光方法,测定细胞半数感染量(TCID50),并与传统鸡胚半数致死量(ELD50)进行相关性分析,相关系数r=0.823。为今后该疫苗样品的病毒含量测定提供了一个新途径,以降低检验成本、缩短检验周期,并提高检验的灵敏度。

Abstract:

The Vero cell was used to establish a cellular immunofluorescence method for evaluation of living infectious bursal disease vaccine (B87 strain). Use the method to determine the amount of virus of the vaccine (TCID₅₀), compared with traditional method (ELD₅₀), and the correlation coefficient r =0.823. If the method is used to evaluate the vaccine, it will reduce inspection costs, shorten the inspection cycle, and improve test sensitivity.