禽流感灭活疫苗（H5N2亚型，D7株）与同类产品对鸭和鸡免疫后血清学比较试验

Comparative test of serology in ducks and chickens immunized by avian influenza vaccine ,inactivated (H5N2 subtype, strain D7) and other similar products

关键词:  禽流感灭活疫苗  D7株  血清学比较试验

Key words:  avian influenza vaccine,inactivated  strain D7  compatitive test of serology

摘要:

为评价禽流感灭活疫苗（H5N2亚型，D7株）与同类产品对不同日龄鸭及SPF鸡的免疫效果，将该疫苗与国内两家禽流感生产企业的2批重组禽流感灭活疫苗（H5N1亚型，Re-5株）分别免疫鸭和SPF鸡，免疫后14、21、28、42 d采血测定各免疫组的HI抗体效价。禽流感灭活疫苗（D7株）以0.25 mL剂量免疫鸭后14 d，所有免疫组鸭的HI抗体几何平均滴度（GMT）即能达到6㏒2以上，28 d HI抗体GMT达到峰值9.1㏒2，而两批重组禽流感灭活疫苗以0.5 mL剂量免疫鸭21 d后HI抗体GMT均没有达到6㏒2；两种疫苗分别免疫21日龄SPF鸡21 d后各免疫组的HI抗体GMT基本相似。试验结果表明，禽流感灭活疫苗（D7株）对鸭的免疫效果要明显优于重组禽流感灭活疫苗(Re-5株)，而对鸡的免疫效果相似。

Abstract:

In order to evaluate the difference of immune effect between the avian influenza virus (AIV) inactivated vaccine (H5N2 subtype, strain D7) and other similar products ,the D7 vaccine and two batches avian influenza inactivated vaccine（H5N1 subtype，strain Re-5）were immunized on different day-old ducks and SPF chicken. The hemagglutinin-inhibition (HI) antibody was detected in 14, 21, 28 and 42 days after immunization . The HI antibody geometric mean titer（GMT）of the duck could reach 6㏒2 more in 14 days after immunized with D7 vaccine , while the HI antibody GMT of the duck couldn’t reach 6㏒2 in 21 days after immunized with the two batch of Re-5 vaccine. The HI antibody GMT of the 21 day old SPF chicken in 21 days after immunized with two group vaccines were basically similar. The results showed that the immune effect on duck of the D7 vaccine was better than that of the Re-5 vaccine, while the immune effect on SPF chicken of the two group vaccines was similar.