IFA和IPMA方法测定猪瘟兔化弱毒病毒含量

IFA and IPMA for HCLV titer detection

关键词:  猪瘟兔化弱毒  TCID50  IPMA  IFA

Key words:  hog cholera lapinized virus  TCID50  IPMA  IFA

摘要:

本研究使用间接免疫荧光方法(IFA)和过氧化物酶单层试验(IPMA)测定猪瘟兔化弱毒的组织半数感染量(TCID50)。优化了两种方法的一抗使用浓度；筛选了IPMA方法中敏感、易辨识的酶作用底物。优化后的IFA和IPMA方法测定猪瘟兔化弱毒疫苗半成品和成品的TCID50,可以作为兔体感染量(RID)和猪体半数保护量(PD50)效力检验标准的补充,应用到猪瘟兔化弱毒活疫苗生产中,加强质检、质控力度。

Abstract:

：Indirect immunofluorescent assay (IFA)and Immunoperoxidase monolayer assay(IPMA) were used to titrate the Tissue Culture Infectious Dose 50(TCID₅₀) of Hog cholera virus lapinized Chinese strain(HCLV). Optimized antibody dilution and Peroxidase Substrate. IFA and IPMA can titer TCID₅₀ of finished and semi-finished products of HCLV vaccines. Though HCLV vaccines were tittered by RID and PD₅₀, we could also titer TCID₅₀ to make sure about vaccines quality . #$NLKeywords: hog cholera lapinized virus ; TCID₅₀；IPMA；IFA