猪轮状病毒的分离与鉴定

Isolation and identification of porcine rotavirus

关键词:  猪轮状病毒  分离  纯净性  特异性  动物回归试验

Key words:  porcine rotavirus  isolate  purity  specification  animal regress experiment

摘要:

将疑似感染猪轮状病毒的内蒙古某猪场的腹泻仔猪粪便样品在MA104细胞上分离培养，得到一株能产生明显细胞病变的毒株（命名为PRV L1株）。经纯净性检测，该毒株无菌生长、无支原体污染及外源病毒污染。特异性检测结果显示该PRV L1株该毒株能被猪轮状病毒单特异性血清中和，并可被猪轮状病毒单克隆抗体识别且在细胞胞浆内观察到典型的绿色荧光，其VP7基因扩增片段序列与G5型猪轮状病毒VP7基因序列相似性高达99%，显示出良好的特异性。动物回归试验结果表明，该毒株口服攻击3日龄仔猪可引起典型的猪轮状病毒病发病症状，并能在发病仔猪小肠内容物中检测到猪轮状病毒。

Abstract:

A strain of porcine rotavirus isolated from the feces of diarrheal piglets in inner Mongolia was replicated serially in MA104 cell cultures, named PRV L1. The purity test indicated that no sterile, no mycoplasma and no extraneous-agents were detected. The specification test manifested that the isolation could be neutralized by anti-rotavirus antiserum and recognized by anti-rotavirus monoclonal antibody, the VP7 gene amplified had nucleotide identities of 99% compared to G5 genotype PRV. The animal regress experiment indicated that the 3 days old piglets fed with the faeces had showed typical syndrome of porcine rotavirus disease, and rotavirus antigen could be detected from intestinal contents.