摘要: |
建立了测定盐霉素预混剂中盐霉素A含量的高效液相色谱分析方法。采用C18色谱柱,甲醇-水-冰醋酸(94︰6︰0.1)等度洗脱,甲醇-硫酸-香草醛(95︰2︰3)为衍生试剂,流速0.7 mL/min;衍生温度98 ℃;检测波长520 nm;进样量20 μL。结果表明,盐霉素A在0.4~0.6 mg/mL范围内线性关系良好(r2=0.9998),平均回收率100.6%。该方法简便、准确、可行。 |
关键词: 高效液相色谱 盐霉素A 含量测定 |
DOI: |
投稿时间:2013-06-06修订日期:2013-07-22 |
基金项目: |
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Determination of Salinomycin A in Salinomycin Sodium Premix by HPLC |
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(Institute of Veterinary Drug Quality Inspection of Shandong Province) |
Abstract: |
A method was established for the determination of salinomycin A in salinomycin sodium premix by high performance liquid chromatography (HPLC). Chromatography was performed on C18 column at 520 nm with the derivative reagent of methanol-sulphuric acid-vanillin(95︰2︰3) and the derivative temperature of 98 ℃. The mobile phase consisted of a mixture of methanol-water-acetic acid (94︰6︰0.1) at a flow rate of 0.7 mL/min. The injected volume was 20 μL. The results showed that there were good linear relations (r2=0.9998) between the concentrations and the peak- areas of salinomycin A with in the range of 0.4~0.6 mg/mL. The average recovery was 100.6%. This method was simple, accurate and feasible. |
Key words: HPLC salinomycin A content determination |