| 摘要: |
| 建立了测定盐霉素预混剂中盐霉素A含量的高效液相色谱分析方法。采用C18色谱柱，甲醇-水-冰醋酸(94︰6︰0.1)等度洗脱，甲醇-硫酸-香草醛(95︰2︰3)为衍生试剂，流速0.7 mL/min；衍生温度98 ℃；检测波长520 nm；进样量20 μL。结果表明，盐霉素A在0.4～0.6 mg/mL范围内线性关系良好（r2=0.9998），平均回收率100.6%。该方法简便、准确、可行。 |
| 关键词: 高效液相色谱 盐霉素A 含量测定 |
| DOI： |
| 投稿时间：2013-06-06修订日期：2013-07-22 |
| 基金项目: |
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| Determination of Salinomycin A in Salinomycin Sodium Premix by HPLC |
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| (Institute of Veterinary Drug Quality Inspection of Shandong Province) |
| Abstract: |
| A method was established for the determination of salinomycin A in salinomycin sodium premix by high performance liquid chromatography (HPLC). Chromatography was performed on C18 column at 520 nm with the derivative reagent of methanol-sulphuric acid-vanillin(95︰2︰3) and the derivative temperature of 98 ℃. The mobile phase consisted of a mixture of methanol-water-acetic acid (94︰6︰0.1) at a flow rate of 0.7 mL/min. The injected volume was 20 μL. The results showed that there were good linear relations (r2=0.9998) between the concentrations and the peak- areas of salinomycin A with in the range of 0.4～0.6 mg/mL. The average recovery was 100.6%. This method was simple, accurate and feasible. |
| Key words: HPLC salinomycin A content determination |
