| 摘要: |
| 采用冻存实验筛选出一种冻存存活率为100%的副猪嗜血杆菌保护剂(HPS-d)，通过冷冻干燥实验测定HPS-d冻干存活率为25.94%。利用单因素分析实验优化保护剂HPS-d基质，在HPS-d中添加3种保护剂基质，结果显示：当海藻糖比例为1%、L-精氨酸盐酸盐比例为3%、BHI培养基/保护剂比例为1:2时，冻干存活率达73.65%~77.61%。Plackett-Burman实验设计考察了保护剂8种组分的保护效果，结果显示：D-山梨醇、L-精氨酸盐酸盐和D-海藻糖是影响冻干存活率的显著因素。冷冻干燥验证实验证明优化后保护剂HPS-d平均冻干存活率达74.77%。 |
| 关键词: 副猪嗜血杆菌 冻干保护剂 存活率 |
| DOI： |
| 投稿时间：2013-05-15修订日期：2013-07-23 |
| 基金项目: |
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| Research on the Cryoprotectant of Haemophilus parasuis |
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| (China Institute of Veterinary Drug Control;China) |
| Abstract: |
| A protective formula, HPS-d was obtained with 100% cryopreservation survival rate via the cryopreservation screen. Further analyzed after vacuum freeze-drying, this HPS-d has the survival rate of 25.94%.HPS-d was optimized by single factor experiment. It is indicated that the lyophilization survival rate can reach 73.65%~77.61%, by adding 3% D- sorbitol, 1% trehalose, 3% sodium glutamate, 1% enzyme hydrolysed casein and 1/2BHI.. By Plackett-Burman design, the three significant factors ,D- sorbitol, sodium glutamate and trehalose ,was confirm with 11 factors as the investigated components and cell survival rate as the target response. Freeze-dried verification tests showed that the average lyophilization survival rate was 74.77%. |
| Key words: Haemophilus parasuis formula of freeze-drying stabilize survival rate |
