| 摘要: |
| 建立了同时测定动物尿液中23种?-受体激动剂的高效液相色谱-串联质谱检测法。样品用2 mol/L盐酸酸解，经SLS固相萃取柱净化、富集后，以甲醇和0.2%甲酸水溶液为流动相进行梯度洗脱，采用多反应监测模式进行定性和定量分析。23种?-受体激动剂的定量下限（LOQ）为0. 1~1.0 μg/L。用猪、牛及羊尿样为研究对象，考察方法的准确度及精密度。23种?-受体激动剂在0.5 μg/L、1.0 μg/L和10 μg/L三个水平下的平均回收率为68%～115%，批内相对标准偏差为0.5%～9.6%，批间相对标准偏差0.6%~14.1%。结果表明，该方法具有准确、简便、灵敏，重现性好等特点，能满足?-受体激动剂类药物的残留检测任务。 |
| 关键词: 动物尿液 b-受体激动剂 高效液相色谱-串联质谱法 |
| DOI： |
| 投稿时间：2013-05-13修订日期：2013-08-19 |
| 基金项目:公益性行业专项（201203023-4） |
|
| Determination of 23 β-agonists in Animal Urine by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
|
| (Zhejiang Provincial Supervisory Institute of Veterinary Drug) |
| Abstract: |
| A high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the determination of twenty-three β-agonists in animal urine. The 2 mol/L hydrochloric acid was used to acidify the sample, and then the sample was purified and concentrated by an SLS SPE column. The separation of the β-agonists was performed on a HPLC system, which was using gradient elution with methanol and 0.2% formic acid. Qualitative and quantitative analysis of the 23 β-agonists were carried out in multiple reactions monitoring mode (MRM). The limits of quantitation (LOQs) of 23 β-agonists were in the range of 0.1-1.0 μg/L. The spiked swine, bovine and goat urine were used to investigate the exactitude and accuracy of this method. The results showed that when the concentrations of the 23 β-agonists were between 0.5 and 10 μg/L, the average recoveries ranged from 68% to 115%, the CV for within-run assays and between-run assays were 0.5%-9.6% and 0.6%-14.1%. The method was veracious, sample, sensitive and repeatable, and it was well suitable for the determination of β-agonists in animal urine. |
| Key words: animal urine β-agonist LC-MS/MS |
