| 摘要: |
| 研究旨在采用星点设计-效应面优化法优选女贞子多糖的最佳提取工艺条件。以浸泡时间、液料比和提取时间为考察因素，多糖提取率作为评价指标，并进行二项式拟合，描绘三维效应面，从效应面上优选较佳的实验条件。结果显示，二项式拟合复相关系数R2 为0.775，女贞子多糖最佳提取工艺为加12倍量水浸泡90 min，提取110 min。该工艺为提高女贞子多糖的提取率提供了实验基础，并为进一步的工业化生产提供实验依据。 |
| 关键词: 女贞子多糖 星点设计 效应面 提取工艺 |
| DOI： |
| 投稿时间：2013-04-24修订日期：2013-06-28 |
| 基金项目:湖南农业大学东方科技学院大学生创新性试验计划项目(DFCXY201234) |
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| Study on the Extraction Process of Ligustrum lucidum Polysaccharide by Central Composite Design-Response Surface Methodology |
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| (Hunan Agricultural University East Science and Technology Faculty) |
| Abstract: |
| The extraction process of Ligustrum lucidum polysaccharide was optimized by central composite design-response surface methodology. Soaking time, solvent content and extraction time were chose as influence factors. The extraction ratio of Ligustrum lucidum polysaccharide was chose as the evaluation index. Then a better extraction process was determined according to binomial fitting and 3D-response surface graphs. The results showed the regression coefficient of the nonlinear estimation was 0.775. The optimized extraction conditions were as following: soaked in 12 times water (liquid-solid rate at 12∶1) for 90 minutes and extracted for 110 minutes twice. The optimum extraction technology by central composite design-response surface methodology provided experimental basis for improving the content of Ligustrum lucidum polysaccharide and experiment basis for its industrial production. |
| Key words: Ligustrum lucidum polysaccharide central composite design response surface extraction process |
