沉淀法分离硫酸粘杆菌素工艺研究

The technology research of separating Colistin sulfate by precipitation

关键词:  硫酸粘杆菌素  沉淀分离法  碱性多肽

Key words:  colistin sulfate  precipitation separation  basic peptides

摘要:

研究了碱性沉淀分离法提取发酵液中多粘菌素E（Colistin sulfate）的生产工艺。通过对沉淀结晶过程pH、温度、结晶速率、晶种量及加入时间、保护剂浓度等工艺参数对结晶效果的影响进行考察。在最佳结晶条件pH11.5-12.0、15-20℃、静置0.5-1h，最高收率达到95%以上，脱色后干粉各项指标达到CP和EP标准。进一步通过中试试验对各个参数进行检验以及校正，得到了一种适用于工业化生产的硫酸粘杆菌素E分离工艺。该方法与目前生产中广泛应用的树脂提取法相比，成品质量及收率明显提高，同时减少了污水排放，环保效益明显。

Abstract:

Study the production process of extracting Colistine sulfate E from fermentation liquid by alkaline precipitation separation method. pH, temperature, crystallization rate, crystal seed of quantity and adding time, protective agent concentration of process parameters during precipitation and crystallization on the effects of crystallization were investigated. Stand for 0.5-1 hour at the best crystallization conditions of pH11.5-12.0 and 15-20℃, the highest yield reached above 95%, bleaching powder after each index to CP and EP standard. After decolorization, indexes of the dry powder can reach CP and EP standard. Further inspection and calibration were conducted by pilot test. A kind of Colistin sulfate E separation technology complies with industrialization production is obtained. Compared with the resin extraction method which is widely applied currently, there is obvious enhancement of quality and yield. Meanwhile, sewage are decreased and the environment effect is obvious.