高效液相色谱法测定阿苯达唑的含量

Determination of Albendazole by HPLC

关键词:  阿苯达唑  高效液相色谱法  质量控制

Key words:  albendazole  HPLC  quality control

摘要:

为建立高效液相色谱法测定阿苯达唑含量，实验优化出最佳色谱条件：检测波长为295 nm，流动相为甲醇﹕水=70﹕30，流速0.8 mL/ min，色谱柱为Shim-pack VP-ODS(150 mm?4.6 mm，5 μm)；柱温为室温。结果显示：阿苯达唑的浓度在1~1000 μg/ mL范围内，峰面积与浓度呈良好的线性关系，R2=1；加样平均回收率为98.1﹪，RSD为0.5﹪；检出限和定量限分别为0.03 μg/ mL和0.1 μg/ mL。该方法简便、快速、准确、重现性好，可用于阿苯达唑的含量测定及质量控制。

Abstract:

To establish the method for determination of albendazole by HPLC, the optimum condition was studied: Detection wave length was 295 nm, the mobile phase was methanol-water (70﹕30), the flow rate was 0.8 mL/ min, the column was Shim-pack VP-ODS(150 mm?4.6 mm, 5 μm), worked at room temperature. The results demonstrated that the peak area and concentration of albendazole in the range of 1~1000 μg/ mL showed a good linear relationship with R2 of 1. The average recovery rate was 98.1﹪with RSD of 0.5﹪. The limits of detection and quantitation were 0.03 μg/ mL and 0.1 μg/ mL, respectively. The method is convenient, rapid, accurate and reproducible, it can be used to detect and control the quality of albendazole.