超高效液相-串联质谱法测定鸡肉组织中金刚烷胺残留

Determination of Amantadine Residues in Chicken Tissue by UPLC-MS/MS

关键词:  金刚烷胺  残留  超高效液相-串联质谱法  鸡肉组织

Key words:  amantadine  residues  UPLC-MS/MS  chicken tissue

摘要:

建立了鸡肉组织中金刚烷胺残留检测的UPLC-MS/MS方法。采用三氯乙酸∶乙腈（1:1,V/V）提取试样中残留的金刚烷胺，SPE净化浓缩，ACQUITY UPLCTMBEH C18色谱柱为分离柱，正离子扫描上机测定。结果显示，金刚烷胺在1~20 ng/mL的范围内线性关系良好（R2=0.997）;样品检出限为1 ng/g，定量限为2 ng/g。在2、5、10 ng/g添加水平的回收率为85%～125%，批内批间变异系数均小于20%。本方法快速、灵敏、重现性好，适用于鸡肉中金刚烷胺的残留检测。

Abstract:

A mothod was established for determination of amantadine residues in chicken tissue by UPLC-MS/MS. Samples were extracted by acetocaustin- acetonitrile(1:1,V/V).Sample purification and concentration were performed by SPE with watersoasis mcx column. Then the reconstituted sample solution was analyzed by UPLC-MS/MS under positive ion mode, with ACQUITY UPLCTMBEH C18 as the analytical column. The results showed that the good linear range was 1.0～20 ng/mL（R2=0.997）. The detection limit was 1 ng/g，and the quantitation limit was 2 ng/g. The recoveries ranged from 85%～125% and the relative standard deviations were below 20% following analysis of spiked at concentrations 2, 5, 10 ng/g. The mothod was reliable, sensitive and reproducibility, adapts to the determination of amantadine residues in chiken tissue.