两株副猪嗜血杆菌的分离鉴定及药敏试验

Isolation, identification and drug sensitivity research ofHaemophilus parasuis 2 strains

关键词:  副猪嗜血杆菌  分离  鉴定  PCR  血清型  药敏试验

Key words:  Haemophilus parasuis  isolation  identification  PCR  serotypes  drug sensitivity test

摘要:

从疑似副猪嗜血杆菌病发病猪场采集58份病料,分离到2株革兰氏阴性细小杆菌。对分离菌株进行培养特性、生化特性、血清型分型研究以及PCR检测和测序分析,结果表明,分离的两株细菌均为血清4型副猪嗜血杆菌。对分离菌株进行药敏试验分析,结果显示分离株对头孢噻肟、头孢噻吩、替米考星高敏,对四环素、丁胺卡那、恩诺沙星、环丙沙星中敏,对甲氧苄氨嘧啶、复方新诺明、链霉素、阿莫西林、新霉素均耐药。

Abstract:

The two small Gram-negative bacilli were isolated from 58 samples of pigs with suspected infection by Haemophilus parasuis in Henan . Isolates were identified as Haemophilus parasuis with the culture characteristics, biochemical method ,PCR and sequecing. 2 isolates were serotyped by KieLetein-Rapp-Gabriedsonas as serotype 4. Results of drug sensitivity test showed that isolates were strong resistance to trimethoprim, sulfamethoxazole, streptomycin, amoxicillin, neomycin , highly sensitive to ceftiofur, cefotaxime, tilmicosin and sensitive to tetracycline, amikacin, enrofloxacin, ciprofloxacin.