高效液相色谱法检测动物脂肪组织中辛硫磷残留量的研究

Study on Determination of Phoxim Residues in Animal Adipose Tissue by HPLC

关键词:  辛硫磷  高效液相色谱法  脂肪组织  残留

Key words:  phoxim  high performance liquid chromatography  adipose tissue  residues

摘要:

建立了高效液相色谱法检测动物脂肪组织中辛硫磷残留量的方法。均质后的动物脂肪组织在微波液化后，经乙腈提取,正己烷脱脂,旋转蒸发浓缩后净化, 残余物用80％乙腈-水溶解，供高效液相色谱定量（紫外检测器）检测。该方法在浓度0.05～2 μg/mL之间呈线性相关,相关系数(R2)为0.9999；在空白脂肪组织样品中添加20（定量限）、200、400、800 μg/kg的辛硫磷,回收率为80%～110%,相对标准偏差(RSD)小于10%。该方法简单快速,灵敏度高,重现性好,适合动物组织中辛硫磷残留量的测定。

Abstract:

In this study，the high performance liquid chromatography on the detection of phoxim residues in animal adipose tissue was established. The animal adipose tissue was liquefied by microwave and the phoxim residuals in animal adipose tissue was extracted by acetonitrile with n-hexane.Extiacts were concentrated to dryness by rotary evaporator and then was purified. The residues was dissolved by 80% acetonitrile-water, detected by high performance liquid chromatography, quantified by the external standard method.In the test, the linear correlation coefficient was 0.99998 within standard solution concentration 0.05~2 μg/mL.The recovery rate was 80%~110% and the relative standard deviation was less than 10%, when phoxim was added by 20，200，400，800 μg/kg in blank samples.The experiment showed that the method was simple, rapid, high sensitivity, good reproducibility and suitable for detection of phoxim residues in animal adipose tissue.