| 摘要: |
| 对市场销售的四种未明确标识可用于猪尿样品的赛庚啶ELISA试剂盒进行考察，使用空白猪尿和不同浓度的空白添加猪尿对其反应原理、可操作性、灵敏度、检测限、回收率等进行研究，发现其中三种赛庚啶ELISA试剂盒可用于猪尿中赛庚啶残留检测，但需要根据试剂盒的特点按需选择。结果表明使用市售的ELISA试剂盒监测猪尿中赛庚啶残留简便可靠，可作为初筛方法，检测限为0.5μg/L。 |
| 关键词: 赛庚啶 残留 猪尿 酶联免疫试剂盒 |
| DOI： |
| 投稿时间：2012-08-20修订日期：2013-03-07 |
| 基金项目:上海市地方标准制修订项目（沪质技监标[2012]309号） |
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| Study for the Detection of Cyproheptadine Residue in Swine Urine by ELISA Kits |
|
| (Shanghai Municipal Supervisory Institute Veterinary Drugs and Feedstaff) |
| Abstract: |
| In this paper, four kinds of ELISA Kits for Cyproheptadine detection（signs for urine）were respectively studied by evaluating and testing some properties including reaction principle, operability, sensitivity, limit of detection and recovery，by blank swine urine and adding with different concentration of CYP. The results showed that three of the kits had high sensitivity and reliability, however, the kits would be chosen according to the properties. We demonstrate that Cyproheptadine ELISA kits could be used as screening method to detect Cyproheptadine residue in swine urine for their operability and applicability, the LOD of Cyproheptadine ELISA kits were suggested to estimate as 0.5μg/L. |
| Key words: Cyproheptadine residue swine urine ELISA kits |
