| 摘要: |
| 本文建立高效液相色谱法检测白龙散、三子散、四味穿心莲散中非法添加土霉素的方法。用十八烷基硅烷键合硅胶色谱柱（4.6?150㎜，5μm），以磷酸盐缓冲液:乙腈（84:16）为流动相，检测波长353nm，流速1.5mL/min，进样量10μL，柱温为室温。结果表明土霉素在0.5-1000μg/mL范围内线性关系良好；检测限分别为白龙散0.3μg/mL，三子散1μg/mL，四味穿心莲散0.2μg/mL。本方法的回收率在98.3%-100.2%之间，RSD为0.16%-0.73%。结果表明，本方法准确、可靠，适用于中药散剂中非法添加土霉素含量的检测。 |
| 关键词: 中药散剂 土霉素 高效液相色谱法 |
| DOI： |
| 投稿时间：2012-08-16修订日期：2012-09-26 |
| 基金项目: |
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| Determination of oxytetracycline in Chinese Herbal Powder by HPLC |
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| (Shanxi Province Institute of Feed and Veterinary Drug Control Taiyuan 030027) |
| Abstract: |
| Abstract: An HPLC method was established for effective detection on oxytetracycline added illegally in Chinese Herbal powders as Bailong San, Sanzi San and Siweichuanxinlian San, etc. A reverse – phase C18 column was used, the mobile phase was phosphate buffer– acetonitrile (84:16), the determination wave length was 353nm, the flow rate was 1.5mL/min ,the sample size was 10μL , the column worked at room temperature. The result demonstrated that the linearity range of oxytetracycline was 0.5-1000μg/mL，the limit of detection of Bailong San was 0.3μg/mL,the limit of detection of Sanzi San was 1μg/mL and the limit of detection of Siweichuanxinlian San was 0.2μg/mL.The recovery was 98.3%—100.2%, RSD was 0.16%—0.73%.It indicated that this method was accurate, reliable and suitable in the determination of oxytetracycline added illegally in veterinary medicine powders. |
| Key words: Chinese Herbal Powder oxytetracycline HPLC |
