| 摘要: |
| 为了用HPLC法同时测定复方防己口服液中粉防己碱和防己诺林碱的含量。采用Diamonsil C18柱(5 μm，4.6 mm?250 mm)，以乙腈-0.2%磷酸水溶液－三乙胺(63∶37∶0.05)为流动相，流速为1.0 mL/min，检测波长282 nm的液相检测方法。结果表明，在此色谱条件下，粉防己碱和防己诺林碱的分离良好，分别在20.52～205.2 μg/mL和17.84～178.4 μg/mL浓度范围内峰面积与浓度呈良好的线性关系。按外标法以峰面积计算进行测定，粉防己碱和防己诺林碱的平均回收率分别为98.15%和97.35%，RSD分别为1.39%和1.90%。表明该方法简便，快速，准确，适用于防己散中粉防己碱和防己诺林碱的含量测定。 |
| 关键词: 复方防己口服液 粉防己碱 防己诺林碱 高效液相色谱法 |
| DOI： |
| 投稿时间：2012-08-02修订日期：2012-10-30 |
| 基金项目: |
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| Determination of Tetrandrine and Fangchinoline in Compound Fangji Oral Solution by HPLC |
|
| (Shanxi Institute of veterinary Drug Control) |
| Abstract: |
| A method for the concentration of tetrandrine and fangchinoline in Compound Fangji Oral Solution was developed by high performance liquid chromatography(HPLC). The operating conditions were Diamonsil C18 column (5 μm, 4.6 mm?250 mm) at room temperature, acetontrile-0.2% phosphoric acid solution - triethylamine (63∶37∶0.05) as mobile phase at a flow of 1.0 mL/min and UV detection at 282 nm. The results indicated that calibration curves for tetrandrine and fangchinoline were linear over the range of 20.52～205.2 μg/mL and 17.84～178.4 μg/mL,respectively.The mean recoveries of tetrandrine and fangchinoline were 98.15% and 97.35%,and RSD were 1.39% and 1.90%,respectively.In conclusion,the method is simple, rapid and reliable for the determination of tetrandrine and fangchinoline in Compound fangji oral solution. |
| Key words: HPLC Compound Fangji Oral Solution Tetrandrine Fangchinoline |
