猪瘟阴、阳性血清国家参照品候选物的均匀性考察

Homogeneity Test of Candidate National Reference Materials for Negative and Positive Antisera of Classical Swine Fever

关键词:  猪瘟  参照品  均匀性

Key words:  classical swine fever  reference materials  homogeneity

摘要:

对猪瘟阴、阳性血清国家参考品候选物的均匀性进行检验。每亚批随机抽取16支样品,其中1支样品取样10次,其余样品取样1次,用猪瘟ELISA抗体检测试剂盒测定其OD₄₅₀值。用t 检验和F 检验进行统计分析。结果显示,各组测定数据的均值与方差差异无统计学意义。该批候选物是均匀的。

Abstract:

To test the homogeneity of candidate national reference materials for negative and positive antisera of classical swine fever (CSF), 16 samples were randomly selected from each sub-batch of the candidate, from which one sample was tested 10 times with a CSFV Antibody ELISA Kit to measure the OD₄₅₀ values, the remains tested only once. Statistics researches were conducted with t- test and F- test. It showed that average and variance of within-bottle and between-bottle results of three sub-batches did not showed significant difference. It could be concluded that the candidate national reference materials had appropriate homogeneity.