高效液相色谱法测定鸡鸭羊组织中环丙氨嗪残留量

ZHOU Wei, YING Yong-fei, WEI Ming-jue, LUO Cheng-jiang, ZHU Cong-ying

关键词:  环丙氨嗪  残留  鸡  鸭  羊  组织  高效液相色谱法

Key words:  cyromazine  residues  chicken  duck  sheep  tissues  HPLC

摘要:

建立了高效液相色谱法测定禽、羊组织中环丙氨嗪残留量的方法。动物组织样品中残留的环丙氨嗪经三氯乙酸/乙腈溶液提取，经MCX固相萃取小柱净化后，用高效液相色谱仪测定。方法的检测限和定量限分别为10 μg?kg-1和20 μg?kg-1。羊肉、羊肝、羊肾和羊脂肪在25~600 μg?kg-1添加浓度范围内，回收率为74.4%~107%，批内RSD在0.9%~11.9%之间，批间RSD在0.3%~11.1%之间。鸡肉、鸡肝、鸡脂肪和鸭肉在25~600 μg?kg-1添加浓度范围内，回收率为74.9%~101%，批内RSD在1.9%~10.7%之间，批间RSD在1.2%~7.1%之间。结果表明：本方法能满足动物性食品中环丙氨嗪测定的需要。

Abstract:

A high-performance liquid chromatography (HPLC) method for the determination of cyromazine in poultry and sheep tissues was optimized and validated. The tissues were extracted by trichloroacetic acid/acetonitrile, and a solid-phase extraction (MCX) was followed, finally, the eluate was assayed by HPLC. As the results shows, the limit of detection (LOD) was 10μg?kg-1, and the limit of quantitation (LOQ) was 20 μg?kg-1. Recoveries of cyromazine in different sheep tissues were between 74.4% to 107%, when its additive concentrations were between 25~600 μg?kg-1, the coefficient of variation (CV) for within-run assays were between 0.9.9% and CV for between-run assays were between 0.3%~11.1%. Meanwhile, the recoveries of cyromazine in poultry different tissues were between 74.9% to 101%, when its additive concentrations were between 25~600 μg?kg-1, the CV for within-run assays and CV for between-run assays were between 1.9%~10.7% and 1.2%~7.1%, respectively. It was concluded that this method was simple, sensitive and reliable for food safety.