鸡血浆中黄芩苷及绿原酸的HPLC法建立

Simultaneous quantification of baicalin and chlorogenic acid in chicken plasma by HPLC

关键词:  黄芩苷  绿原酸  血浆  高效液相色谱法

Key words:  Baicalin Chlorogenic acid Plasma High pressure liquid chromatography

摘要:

建立鸡血浆中复方慢呼抗口服液主要成分黄芩苷和绿原酸测定的高效液相色谱法。选择流动相为乙腈、1%冰醋酸,梯度洗脱30min,黄芩苷和绿原酸的波长分别为280nm、327nm,流速1mL/min。采用高氯酸法、甲醇乙腈法、正丁醇法处理血浆,选取最优方法进行考察。甲醇乙腈法处理血浆,方法专属性好,回收率较高,线性关系良好,精密度及准确度较高,可用于药代动力学研究。

Abstract:

To establish the high performance liquid chromatography(HPLC) method for simultaneous quantification of baicalin and chlorogenic acid in chicken plasma of the main ingredient in Manhukang oral solution, and select the optimal plasma treatment. Acetonitrile -1% glacial acetic acid was used as mobile phase , gradient elution lasted for 30min, the wavelength of baicalin and chlorogenic acid was 280nm and 327nm, respectively and the flow rate was at 1.0mL/min. The results showed that using methanol and acetonitrile to process the plasma is the best method which possesses high specificity and high recovery good linear relationship precision and accuracy determination. The improved analytical method was found to be rapid, sensitive and suitable for application in pharmacokinetic studies.