| 摘要: |
| 建立了高效液相色谱法测定创伤愈合药喷雾剂中血竭素含量的方法。色谱柱为C18柱(5 μm，4.6 mm?250 mm)，流动相为乙腈-0.05mol/L磷酸二氢钠溶液（50:50）（用磷酸调pH为3.5）为，流速1.0mL/min，柱温40℃，检测波长440nm。血竭素在13.07～45.74ug/mL浓度范围内，峰面积与浓度线性关系良好，r=0.9997（n=6），平均加样回收率为98.29%，相对标准偏差为0.67%，血竭素的平均含量为0.01902 mg/mL。本研究所建立的含量测定方法简便、灵敏、准确、稳定，可用于创伤愈合药喷雾剂的质量控制。 |
| 关键词: 创伤愈合药喷雾剂 血竭素 HPLC |
| DOI： |
| 投稿时间：2012-04-27修订日期：2012-05-26 |
| 基金项目:四川农业大学本科生“创新性实验”项目 |
|
| Determination of the Dracorhodin Content of Wound Healing Medicine Compound Spray by HPLC |
| 张灵英 |
| (College of Veterinary Medicine Sichuan Agricultural University,Ya’an) |
| Abstract: |
| A method of C18-functionalized magnetic silica nanoparticles solid phase extraction of chloramphenicol residues from shrimp was developed. The reversed-phase extraction dual particles were synthetized by bonding C18 groups on the surface of the superparamagnetic Fe3O4 nanoparticles and were used to extract veterinary drug residues by SPE.The process conditions, such as temperature, uniting time and additive amount of C18-functionalized magnetic silica nanoparticles were optimized. The detection method showed good linear relationship within the range of 0.1～10 μg/kg(R2=0.9941) and the detection limit was 0.1 μg/kg. The recovery range was between 73.3% and 112.3%. The method was reliable，accurate and had a good reproducibility，which can meet the demands of the detection of chloramphenicol residues in shrimp. |
| Key words: wound healing medicine compound spray dracorhodin HPLC |
