| 摘要: |
| 以103.0 EID50、104.0 EID50 和105.0 EID50 3个不同的病毒含量接种非免疫鸡胚,收获鸡胚液,结果显示103.0~104.0EID50病毒含量接种鸡胚收获的抗原含量高。用该优化方法繁殖了一批新城疫抗原,采用截留分子量为100kD的浓缩滤膜对新城疫抗原进行浓缩,对不同浓缩倍数的新城疫抗原制备疫苗进行了免疫效力试验。结果表明以新城疫3倍浓缩抗原制备疫苗,以5μl剂量(现有相关产品效力检验的1/4剂量)免疫SPF鸡,即可达到现有同类产品的质量标准,高抗体水平至少可以维持35天。本研究为进一步研制新城疫灭活疫苗浓缩疫苗用于低剂量免疫肉鸡提供了研究基础。 |
| 关键词: 新城疫抗原 浓缩 灭活疫苗 |
| DOI： |
| 投稿时间：2012-04-24修订日期：2012-07-05 |
| 基金项目:国家科技支撑计划(2009BADB4B05)；科技部科技基础性工作专项(2008FY130100) |
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| Preliminary Study on concentrated inactivated Newcastle disease vaccine |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| Non-immunized chicken embryos were inoculated with newcastle disease viruses in three different virus content. The results showed that the antigen content of chicken embryos inoculated with 103.0~104.0EID50 viruses were higher than 105.0 EID50 inoculated group.A batch of newcastle disease virus was propagated and concentrated with 100 kD filtration membrane for different concentration fold. The immune efficacy of different concentrated antigen inactivated vaccine was analyzed. The vaccine prepared with 3 fold concentrated antigen was inoculated SPF chicken with 5 μL and could induce high antibody titer for at least 35 days. This study provided a research foundation for further development of chicken newcastle disease vaccine. |
| Key words: Newcastle disease virus concentration inactivated vaccine |
