| 摘要: |
| 为摸清猪肺炎支原体的颜色变化单位（CCU）测定是否受试验相关因素的影响，通过不同培养体系、不同种类的容器、不同的稀释方法测定猪肺炎支原体的CCU，观察培养基的变色情况，最终通过测定各组猪肺炎支原体的CCU来分析不同因素对猪肺炎支原体培养滴度的影响。结果显示，利用不同培养体系、不同容器及不同的稀释方法测定猪肺炎支原体的CCU，其结果差异均不显著（P>0.05）。该研究可为今后支原体的CCU测定提供参考。 |
| 关键词: 颜色变化单位 猪肺炎支原体 |
| DOI： |
| 投稿时间：2012-04-09修订日期：2012-07-12 |
| 基金项目:国家自然科学基金（31001080）；江苏省农业自主创新基金［cx(10)427,cx(11)2048］ |
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| Effects of Various Factors on the Color Changing Units Assay of Mycoplasma hyopneumoniae |
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| (Institute of Veterinary Medicine, Jiangsu Academy of Agriculture Sciences) |
| Abstract: |
| The color changing units（CCU） assay of Mycoplasma hyopneumoniae is a method of quantitative study of Mycoplasma, which is the determination of Mycoplasma in classical method. To make sure whether the color changing units assay of Mycoplasma hyopneumoniae is affected by different factors,the test used different culture system ,different types of container, different dilution method to determinate the CCU of Mycoplasma hyopneumoniae, observe the color changing of medium, ultimately determine the CCU to analyze the effect of different factors on the determination of CCU. The results showed that the use of different culture system, different containers and different dilution method for the determination of CCU, which were not significant differences. This test offered a fairly good reference for the color changing units assay of Mycoplasma hyopneumoniae. |
| Key words: color changing units Mycoplasma hyopneumoniae |
