三种布鲁氏菌病疫苗株的毒力比较

violence comparision between three different vaccine strains of brucellosis in mice and guinea pigs

关键词:  布鲁氏菌  疫苗  毒力

Key words:  brucella  vaccine  virulence

摘要:

为了系统比较我国现有布鲁氏菌病疫苗株A19、M5和S2的毒力,本研究分别用上述3种疫苗株以1?105 CFU/只免疫Balb/c小鼠,免疫后每隔2周采集小鼠脾脏,分离细菌,测定各疫苗株在小鼠脾脏中的存留时间。结果A19、M5、S2在小鼠体内存活时间依次为14周、大于16周、6周。将3种疫苗株以1?109 CFU/只分别免疫Hartley豚鼠,15日后测定豚鼠脾脏含菌量,结果A19、M5、S2免疫后每克脾脏含菌量分别为2.8?104CFU、大于6.7?105CFU、3.8?103CFU。本研究结果表明,我国目前使用的布鲁氏菌疫苗中,S2毒力最弱,A19其次,M5最强。

Abstract:

In order to compare the violence of the applied vaccine strains of brucellosis, which are B.abortus A19, B.melitensis M5 and B.suis S2, three groups of above vaccines with the dose of 1?105 CFU each were inoculated to female Balb/C mice,respectively. The specific bacterica were isolated and identified by PCR from the spleens of each group every 2 weeks post inoculation. The suvival tests shows that A19, M5 and S2 strains could stay in spleen for 14 weeks, more than 16 weeks and 6 weeks post inoculation, respectively.The same dose of bacterias were injected to adult female guinea pigs in groups, respectively, and the amount of ioslated bacteria were calculated for each group of spleens per grame. The aomount of containing bacteria for group A19, M5 and S2 were 2.8?104CFU, more than 6.7?105 CFU and 3.8?103CFU. This study shows that in all the three kinds of brucella vaccines, S2 is the weakest violent and M5 is the strongest.