HPLC法测定帕那珠利的含量

Determination of ponazuril content by HPLC

关键词:  帕那珠利  HPLC法  含量测定

Key words:  ponazuril  HPLC  assay

摘要:

建立了帕那珠利原料药含量测定的HPLC法。以0.2%乙酸水溶液-乙腈(43∶57)为流动相，采用Diamonsil C18反相色谱柱（250 mm?4.6 mm，5 μm），柱温35 ℃，流速1 mL/min，紫外检测波长255 nm。在此实验条件下，帕那珠利在10~100 μg/mL浓度范围内与峰面积线性关系良好（r=0.9999，n=5）；精密度良好，RSD为0.25%(n=5)；平均回收率为98.6%，RSD为1.2%（n=9）；重现性良好，RSD为0.69%（n＝5）；样品在24 h内稳定,RSD为0.74%。本方法准确、简便、快速，可以用于帕那珠利的含量测定。

Abstract:

The paper established an HPLC method for determination of ponazuril. The diamonsil C18 column was used; the mobile phase consisted of water (0.2% acetic acid)-acetonitrile(43∶57); the flow rate was 1 mL/min; the column temperature had been remained at 35 ℃; the detection wavelength was 255 nm. The linear range of ponazuril was 10~100 μg/mL (r=0.9999). The mean recovery was 98.6% (n=9). The precision was excellent (RSD=0.25%, n=5) as well as the reproducibility (RSD=0.69%, n=5). The sample was stable enough to be detected in 24 hours (RSD=0.74%). This method is accurate, easy and fast. It can be used to detect and quantify ponazuril.