| 摘要: |
| 通过研究不同类型培养基和培养温度、起始pH值等发酵条件对IL-15产量的影响,初步确认了摇瓶水平发酵各因素的最优条件,优化的发酵条件为发酵起始pH值7.0、培养温度37℃、乳糖诱导浓度1.5g/L、菌体生长密度OD600达到1.0时加入乳糖、诱导时间为4h，此时IL-15产量达到最高水平,从而为IL-15批量生产奠定了良好的基础。 |
| 关键词: IL-15 摇瓶发酵 表达 优化 |
| DOI： |
| 投稿时间：2012-03-03修订日期：2012-03-28 |
| 基金项目:吉林化工学院科研基金(2011-046) |
|
| Optimization of production technology of Recombinant humanInterleukin-15 in Shaking Flask |
| 杨梅 |
| (College of Environment and Bioengineering,Jilin Institute of Chemical Technology,Jilin) |
| Abstract: |
| The expression level of IL-15 was influenced by the different medium and other fermentation parameters including culture temperature, initial pH and so on. The optimal parameters of various factors was confirmed by flask-fermentation.The optimal fermentation parameters with lactose induction is culture medium pH7.0,culture temperature 37℃, joining lactose to final concentration 1.5g/L when the recombinant strain growth density OD600 achieved 1.0 and induction time 4h. The production of IL-15 reached the highest level.This fermentation technology established a good foundation for the IL-15 mass production. |
| Key words: Interleukin -15 flask- fermentation expression optimization |
