| 摘要: |
| 制备了吡喹酮脂质体冻干剂,并对其质量进行评估。采用薄膜-超声法制备吡喹酮脂质体，结合冷冻干燥技术制备吡喹酮脂质体冻干剂，建立HPLC法测定吡喹酮脂质体的包封率，并研究其稳定性。优化后的脂质体冻干剂其包封率在70%以上,粒径范围220～310nm,电镜结果显示,脂质体外观圆整而均匀。本法制备的吡喹酮脂质体冻干剂质量稳定,且建立的包封率测定方法稳定可靠。 |
| 关键词: 吡喹酮脂质体 冻干剂 冷冻技术 包封率 |
| DOI： |
| 投稿时间：2011-11-22修订日期：2012-03-21 |
| 基金项目:长沙市重点科技计划项目(K1005006-21) |
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| Preparation and Assessment of Freeze-dried Praziquantel Liposome Agent |
| DENG Fang,匡光伟 |
| (Hunan Provincial institute of animal Drug and Feed supervision,Changsha) |
| Abstract: |
| The study was designed to establish a method for freeze-dried praziquantel liposome agent preparation, as well as the corresponding quality control and assessment. We initially used the film-ultrasonic wave technique to prepare the praziquantel liposomes, then freeze-drying technique to yield the freeze-dried praziquantel liposome agent, after which the agent stability and the encapsulation efficiency werecarefully assessed and determined via our newly set up HPLC-method. The encapsulation efficiency can be more than 70% with particle size range from 220nm to 310nm, which most liposomes turned out to be round and even to show a better appearance. Stable and high quality freeze-dried praziquantel liposome agent can be obtained with high quality according to our newmethod. And the corresponding established HPLC-method was reliable for the encapsulation efficiency assessment of the liposome agent. |
| Key words: Praziquantel Liposome Freeze-dried agent Freeze Technique Encapsulation efficiency |
