氯霉素化学发光酶联免疫检测方法的建立

Establishment of Chemiluminescent Enzyme Immunoassay for Chloramphenicol

关键词:  氯霉素,化学发光酶联免疫法,试剂盒

Key words:  Chloramphenicol  chemiluminescent enzyme immunoassay  Kit

摘要:

在常规酶联免疫法（ELISA）的基础上，用辣根过氧化物酶标记氯霉素与牛血清白蛋白偶联后免疫小鼠制得的单克隆抗体，并优化抗体工作浓度、反应时间等试验参数，建立了一种简便、快速、准确的氯霉素（CAP）残留一步式化学发光酶联免疫检测方法。结果表明：该方法最佳检测范围为0~1620pg/mL，检测灵敏度达20pg/mL，检测限为10pg/mL，可检出国际规定的CAP残留标准限量值以下的残留量。与常规ELISA检测方法相比，不仅提高了检测限和灵敏度，而且反应时间缩短了60min。证明采用一步式化学发光酶联免疫检测氯霉素残留，可以大幅度缩减检测时间，更符合快速检测的要求。

Abstract:

Based on conventional ELISA ,through use HRP labeled monoclonal antibodies that obtained from immunizing mice that inject with CAP-BSA coupling protein, and optimize test parameters,such as antibody concentration, reaction time, established a convenient, rapid and accurate one step chloramphenicol (CAP) residue chemiluminescent enzyme immunoassay（CLEIA） detection methods. The results show that the best detection range of this method was 0~1620 pg/mL,the detection sensitivity was 20 pg/mL, the detection limit was 10 pg/mL, can check out the international provisions standard limited value of the CAP residue.Compared with conventional ELISA test method, the CLEIA method can not only increase the detection limit and sensitivity, but also shorten the reaction time by 60 minutes. Indicate that using the one step chemiluminescent enzyme immunoassay kit to detect chloramphenicol residue can decrease time consuming, and more appropriate to fast detection requirements.